Unique Expression Systems With One-Of-A-Kind Properties

Autodisplay Technology is Autodisplay Biotech’s first generation cell surface display technology. It enables the display of heterologous proteins and peptides on the surface of E. coli. Therefore, peptides/proteins of choice are anchored (naturally immobilized) in the outer membrane of the bacterial cell (for more information on the Autodisplay mechanism, please scroll to the bottom of the page). Our unique plug&play technology has proven to add significant value to numerous industries such as the pharmaceutical, diagnostics and cosmetic industries.

 

Autodisplay Features

  • Between 100.000 and 200.000 molecules per cell
  • Natural immobilization and stabilization on the cell surface
  • Multimerisation/aggregation of protein complexes is possible (homomers and heteromers)
  • Incorporation of an inorganic prosthetic group into an apoprotein on the cell surface
  • Up to three different proteins/protein domains on the surface of one strain

 

We took our very own and made it even better:
MATE - the second generation Autodisplay Technology

 

Recently, we have developed an improved cell surface display technology called MATE (maximized autotransporter expression). This latest technology from Autodisplay Biotech comes with a novel autotransporter and extends the application of surface display from E. coli to other Gram negative bacteria. But that's not all - when we designed MATE, we incorporated other very useful features which enable a particularly easy secretion and purification of any protein of interest. Because MATE retains all characteristics of Autodisplay Technology, our new technology can be seen as an upgrade with even broader application possibilities.

 

Application Fields For Autodisplay

Autodisplay’s technologies add significant value to processes in a broad range of industries such as the pharmaceutical, diagnostics, cosmetics, chemical, food and biotechnology industries. Currently, Autodisplay Biotech GmbH has set a focus on three application fields:

 
Our whole cell biocatalysts comprise all advantages of free enzymes and immobilized enzymes:

  • No limitations to substrate or product (e.g. size, toxicity)
  • Easy product purification because enzymes are bound to bacterial beads
  • Recovery re-use of whole cell biocatalysts through filtration
  • Ability to run continuous processes in oppose to batch processes

 
We functionalize solid surfaces for bioanalytical tools (e.g. SPR Biochips, ELISAs) and separation/detox materials. Our expression systems offer numerous advantages:

  • Simplifying the development of customized tools and materials
  • High level of cost-efficiency through natural immobilization of target molecules
  • Enhanced sensitivity of biochips through full orientation control at cell membrane

 
High-throughput screening for binding interactions or enzyme activity with the following advantages:

  • Compatibility with advanced screening methods such as FACS and therefore faster results
  • Simplified enzyme optimization through target molecule’s location on cell surface
  • Rapid isolation of improved variants due to self-replication (direct connection between function and gene)

 
 

Further Information on the Technology

The autodisplay system was developed based on the secretion mechanism of the autotransporter family of proteins. Gram negative bacteria have evolved the autotransporter pathway in order to transport proteins to the cell surface or secrete proteins into the extracellular milieu. This mechanism is outstanding in its apparent simplicity.

 

The Autodisplay Mechanism

Autotransporter proteins are synthesized as multidomain proteins containing all structural requirements for the transport to the cell surface. They contain an N-terminal signal peptide typical for the Sec pathway, enabling export across the inner membrane. After truncation of the signal peptide in the periplasm, the C-terminal domain folds into the outer membrane as a porine-like structure, a so called ╬▓-barrel. The passenger domain can be efficiently translocated to the extracellular milieu by this structure. Recombinant passengers can be transported to the surface by simple insertion of the corresponding coding sequence into a distinct position of the precursor gene.

 

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